Figure 1: (a) Design and synthesis of the pET-21a-Ina_K 537-TEV-VEvhh10 construct. (b) Schematic representation of the pET-21a plasmid containing Ina_K 537, linearly designed according to cuts with HindIII and XhoI enzymes. The final product (TEV-VEvhh10) from PCR, with an added site for TEV protease enzyme cleavage before the VEvhh10 sequence, resulted in a length of 402 bp. (c) Schematic representation of the surface expression of VEvhh10 using the ice nucleation protein in the E. coli host.