Fig. 3: a) Plasmid pET28a after double digestion using the cutting enzymes BamHI and XhoI. Purification of the product. b) Products of the PCR reaction SUMO with the enzyme pfu after double digestion using the cutting enzymes HindIII and BamHI and purifying the product. c) Products of the PCR reaction VEvhh10 with the enzyme pfu after double digestion using the cutting enzymes HindIII and XhoI and purifying the product.